EIA HSV 1+2 IgA

Enzyme immunoassay for the detection of IgA antibodies against Herpes simplex virus of type 1+2 in human serum or plasma.

Description:

• Microtitre wells are coated with the inactivated and purified strains of HSV-1 and HSV-2 as an antigen.
• If present, specific antibodies bind to the antigen. The complex is labelled with Conjugate and detected through a colour reaction with substrate (TMB-Complete).
• The kit contains 96 tests (including controls) in a microtitre plate with colour-coded strips and breakable wells.
• Total assay time: approximately 1.5 h.
• High sensitivity and specificity.
• Colour reagents for easy pipetting.
• The kit may be used sequentially for smaller batches of samples, reagents are provided in sufficient quantities.
• CUT-OFF included.
• Semiquantitative evaluation using the index of positivity (IP)
• Controls, Conjugate and substrate (TMB-Complete) are supplied in working strength.
• Identical (i.e. interchangeable) Sample diluent, substrate TMB-Complete, Washing solution, Stopping solution in the EIA HSV 1+2 kits.
• Expiry period: 12 months.

Applications:

• Searching test for detection of human infection Herpex simplex
• Checking of therapy results using the semiquantitative determination

Brief assay procedure:

1. Dilute samples (1+100).
2. Pipette controls and diluted samples.
3. Incubate for 30 min at 37 °C.
4. Aspirate and wash the wells 5 times.
5. Add Conjugate.
6. Incubate for 30 min at 37 °C.
7. Aspirate and wash the wells 5 times.
8. Add substrate (TMB-Complete).
9. Incubate for 30 min at 37 °C.
10. Add Stopping solution (H₂SO₄).
11. Read photometrically at 450 nm.
12. Evaluate results.

Safety Sheets