BLOT-LINE ENA plus

BLOT kit for the detection of specific IgG antibodies to the recombinant extractable nuclear antigens and centromere B in human serum or plasma

Description:

• Used antigens: SS-A/Ro 52, SS-A/Ro 60, SS-B/La, Scl-70, RNP-A, RNP-C, RNP-68, SmB, SmD, Jo-1 and centromere B.
• The recombinant antigens are applied to the nitrocellulose membrane (i.e. to the respective BLOT‑LINE (BL) strips).
• If specific antibodies are present in the sample, they will bind to the respective antigens.
• The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
• The kit contains 20 tests.
• Short incubation periods, total assay time: approximately 1.5 h.
• High sensitivity and specificity.
• All reagents are supplied in working strength.
• The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
• All BL strips are equipped with the CUT-OFF band to check functionality and sensitivity of the kits.
• Positive and Negative controls included enabling validation of the tests.
• Blotting trays included.
• Evaluation of results – clear criteria using Evaluation table.
• Software evaluation of results is also possible – IMUNOBLOT II software (TEST-LINE).
• Expiry period: 12 months from the production date.

Applications:

• Laboratory test for the detection of antinuclear antibodies, confirmatory test to ELISA.

Brief assay procedure:

1. Dilute serum/plasma samples (1:50)
2. Pipette diluted samples and controls into the channels of the tray and insert the BL strips.
3. Incubate for 30 min at room temperature using a shaker.
4. Wash 3 times for 5 min.
5. Add Conjugate.
6. Incubate for 30 min at room temperature using a shaker.
7. Wash 3 times for 5 min.
8. Add substrate (BCIP/NBT).
9. Incubate for 15 min at room temperature using a shaker.
10. Wash twice for 5 min in distilled water.
11. Dry the strips and evaluate results using the validation strip enclosed or using IMUNOBLOT II software.

Safety sheets