BLOT-LINE Borrelia/HGA IgM

BLOT kit for the detection of specific IgM antibodies to the recombinant antigens of Borrelia species and Anaplasma phagocytophila (HGA) in serum, plasma and cerebrospinal fluid

Description:

• Antigen used: combination of selected parts of the specific antigens of Borrelia sp. (VlsE B. afzelii, VlsE B. garinii, VlsE B. b. sensu stricto, p83, p39, OspB, OspA, OspC and p17) and high specific antigen of Anaplasma phagocytophila (p44)
• The recombinant antigens are transferred to the nitrocellulose membrane (i.e. to the respective BLOT‑LINE (BL) strips).
• If specific antibodies are present in the sample, they will bind to the respective antigens.
• The complex is labelled with Conjugate and detected through a colour reaction with substrate (BCIP/NBT).
• The kit contains 20 tests.
• Short incubation periods, total assay time: approximately 1.5 h.
• High sensitivity and specificity.
• All reagents are supplied in working strength.
• The kit may be used sequentially for smaller batches of samples (reagents are provided in sufficient quantities).
• BL strips are equipped with the CUT-OFF band to check functionality and sensitivity of the kits and facilitate evaluation of the test.
• Positive and Negative controls included enabling validation of the tests.
• Colour identification in compilance with antibody classes.
• Blotting trays included.
• Identical (i.e. interchangeable) reagents (except for Conjugate) in all BLOT and BLOT-LINE Borrelia kits.
• Evaluation of results – clear criteria using Evaluation table.
• Software evaluation of results is also possible – IMUNOBLOT II software (TEST-LINE).
• Expiry period: 12 months from the production date

Applications:

• Laboratory test for the detection of Lyme borreliosis, confirmatory test to ELISA.
• Laboratory test for the detection of Anaplasmosis

Brief assay procedure:

1. Dilute serum/plasma samples (1:100), cerebrospinal fluids (1:1)
2. Pipette diluted samples and controls into the channels of the tray and insert the BL strips.
3. Incubate for 30 min at room temperature using a shaking apparatus.
4. Wash 3 times for 5 min.
5. Add Conjugate.
6. Incubate for 30 min at room temperature using a shaking apparatus.
7. Wash 3 times for 5 min.
8. Add substrate (BCIP/NBT).
9. Incubate for 15 min at room temperature using a shaking apparatus.
10. Wash 2 times for 5 min in distilled water.
11. Dry the strips and evaluate results using the validation strip enclosed or using IMUNOBLOT II software.

Safety Sheets